Journal: Journal of Family & Reproductive Health

Article Title: Effect of Lysophosphatidic Acid on the Vascular Endothelial Growth Factor Expression in Autotransplanted Mouse Ovaries Encapsulated in Sodium Alginate

doi: 10.18502/jfrh.v15i2.6449

Figure Lengend Snippet: The sequences of the designed primers

Article Snippet: The cells were permeable by putting the sections in triton X100 (0.3 % for 30 min), washed in PBS and blocked with goat serum (30 min) then, they were incubated with the primary anti-VEGF polyclonal antibody (1:100, Elabscience Biotechnology Co, Wuhan, China) overnight.

Techniques: Sequencing

Journal: Journal of Family & Reproductive Health

Article Title: Effect of Lysophosphatidic Acid on the Vascular Endothelial Growth Factor Expression in Autotransplanted Mouse Ovaries Encapsulated in Sodium Alginate

doi: 10.18502/jfrh.v15i2.6449

Figure Lengend Snippet: The fluorescent microscopy observations of transplanted mouse ovarian tissue sections immunostained for VEGF antibody (first row) and phase contrast of the same groups in second row. A and a: Intact control group; B and b: LPA+/LPA-; C and c: LPA-/LPA-. Green color shows the positive cell reaction (white arrow) for VEGF antibody (Bar=50μm). The comparison of the relative expression ratio of Vegf gene to β- actin in transplanted mouse ovaries and intact control group are shown in parts D.

Article Snippet: The cells were permeable by putting the sections in triton X100 (0.3 % for 30 min), washed in PBS and blocked with goat serum (30 min) then, they were incubated with the primary anti-VEGF polyclonal antibody (1:100, Elabscience Biotechnology Co, Wuhan, China) overnight.

Techniques: Microscopy, Control, Comparison, Expressing

Journal: Journal of Ovarian Research

Article Title: MSCs–derived EVs protect against chemotherapy-induced ovarian toxicity: role of PI3K/AKT/mTOR axis

doi: 10.1186/s13048-024-01545-7

Figure Lengend Snippet: Effect of MSCs-EVs, rapamycin, and quercetin on ( A ) phosphorylation of mTOR, PI3K, and AKT as detected by western blot analysis, ( B - D ) Intensity of immunoreactivity of selected proteins as quantified by densitometry. Results are expressed as mean ± SEM. **significant vs. Control group at p<0.01, **** at p<0.0001, # significant vs. OF group at p<0.05, ## at p<0.01, ### at p<0.001, #### at p<0.0001, & significant vs. OF + Rapamycin group at p<0.05

Article Snippet: Next, the blots were incubated with the appropriate primary antibodies for an entire night at 4 °C, PTEN (E-AB-63495, Elabscience, USA), FOXO3 (NBP2-16521, Novus Biologicals USA), mTOR (sc-517464, Santa Cruz Biotechnology, USA), Phospho-mTOR (sc-293133, Santa Cruz Biotechnology, USA), PI3K (E-AB-64202, Elabscience, USA), Phospho-PI3K (E-AB-20966, Elabscience, USA), AKT (E-AB-15441, Elabscience, USA), Phospho-AKT (E-AB-20804, Elabscience, USA), β-actin (E-AB-20031, Elabscience, USA).

Techniques: Phospho-proteomics, Western Blot, Control

Journal: Autophagy

Article Title: Activation of PPARA-mediated autophagy reduces Alzheimer disease-like pathology and cognitive decline in a murine model

doi: 10.1080/15548627.2019.1596488

Figure Lengend Snippet: Primary antibodies used in this study.

Article Snippet: Rabbit polyclonal anti-SQSTM1 , Elabscience , EAP3350 , 1:1000 , - , -.

Techniques: Western Blot, Immunofluorescence, Immunohistochemistry